AION
The new standard in confocal micrcoscopy
Root of A. thaliana. Nucleus – DAPI, Histone H3 – Alexa488, Tubulin – Cy3. Scale bar 50um.
The most gentle
3D live cell imaging
AION is the third generation of our fast Line REscan confocal technology. It provides high contrast images from thicker specimens such as organoids, tissue samples, plant and animal model organisms. The gentle conditions for your live samples allow AION to excel in long time lapse experiments and imaging low signal samples.
The combination of camera based detection and a set of filters allows you to acquire your images in both visible (VIS) and near-infrared (NIR). This gives you an improved temporal resolution, high sensitivity and unprecedented signal-to-noise ratio. You get even faster imaging with cleaner results, thanks to additional motorized single-band emission filters.
Double the FOV
Double the observation
AION is a confocal system that allows a 25 mm field of view (FOV) with a motorized pinhole that ensures spot on confocality every time.
Our system uses a line illumination through a slit pinhole, instead of a point illumination found in point scanner confocal microscopes. This provides high temporal resolution, while at the same time reducing phototoxicity. Since phototoxicity is one of the biggest challenges for researchers, this makes AION the perfect tool for live cell imaging.
You can also add AION to any widefield microscope. This will turn it into an advanced fast scanning confocal imaging system.
Fixed human fibroblast cells imaged with 100x objective. Nucleus – DAPI (blue), mitochondria – Tom20-Alexa488 (green), microtubules – Tubulin-Alexa555 (red). Scale bar 20 μm.
Accelerate your research
with the AION
- Double FOV size (FN 25).
- Double acquisition speed (over 50 fps).
- Double available laser lines (VIS+NIR).
- Sixteen times more information acquired.
- Unprecedented signal-to-noise ratio.
- The least phototoxic confocal system on the market.
AION technical specs
AION | α | λ | Σ |
|---|---|---|---|
FOV | 25 mm (FN25); 450×450 μm (40x objective) | ||
Resolution in real time | 170 nm with deconvolution; 240 nm raw | ||
Speed | >50 fps @ 3Kx3K | ||
Speed in fixed line mode | >1000 fps (camera limited) | ||
Wavelengths | 400-1100 nm (VIS + NIR) | ||
Detector & Sensitivity | sCMOS ; >95% QE | ||
Software | Micromanager, SDK available for integration on request | ||
Deconvolution | Microvolution (real time); SVI Huygens (post processing) | ||
Modalities | Brightfield, Widefield and Confocal | ||
Widefield/confocal switch | Motorized | ||
Camera ports | 1 | 2 | 2 |
Laser fiber inputs | 1 | 2 | 2 |
Pinhole | Pre-selected | Motorized | Motorized |
Dichroic | Pre-selected | Pre-selected | Motorized |
